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KMID : 0861020100250040103
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Korea Journal of Herbology
2010 Volume.25 No. 4 p.103 ~ p.112
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Anti-oxidative and anti-inflammatory effect of Do-Ki-Tang methanol extract in mouse macrophage cells
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Kim Dong-Wan
Yun Hyun-Jeong
Heo Jun-Young
Kim Tae-Hoon
Cho Hyun-Jin
Park Sun-Dong
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Abstract
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Objective: The aim of this study was to determine whether methanol extract of Do-Ki-Tang (DKT) inhibit free radical generation and production of nitrite an index of NO, PGE2, iNOS, COX-2 and pro-inflammatory cytokines such as TNF-α, IL-1β, IL-6 and MCP-1 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages.
Methods: Cytotoxic activity of extract on RAW 264.7 cells was measured using 5-(3-caroboxymeth -oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The expression level of inflammatory response-related proteins was confirmed by western blot. The production of proinflammatory cytokines was measured by ELISA.
Results: Our results indicated that DKT scavenged DPPH radical and nitric oxide in vitro. Moreover, DKT significantly inhibited the LPS-induced NO, PGE2 production and iNOS, COX-2 expression accompanied by an attenuation of TNF-α, IL-1β, IL-6 and MCP-1 formation in macrophages. Furthermore, DKT treatment also blocked LPS-induced intracellular ROS production and the activation of NF-κB and MAPKs.
Conclusion: Our data suggest that the anti-inflammatory effect of DKT is mediated through down-modulation of pro-inflammatory mediators and cytokines by blocking the signaling pathways of NF-κB and MAPKs. These inhibitory effects by DKT represent a potential therapeutic approach to the treatment of inflammatory diseases.
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KEYWORD
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Do-Ki-Tang (DKT), inflammation, macrophage
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